Compositional Fractionation of Copoly(styrene/methyl methacrylate) by Normal-Phase and Reversed-Phase High-Performance Liquid Chromatography

Reversed-phase high-performance liquid chromatography.

Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column

The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...

Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column

The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...

Separation of actinomycins and biosynthetic analogues by normal and reversed-phase high performance liquid chromatography.

Chromatographic procedures for the separation and identification of actinomycins have been reviewed1}. Only one report of HPLC has appeared2); this described reversed-phase separations of the C complex and of a biosynthetic mixture of actinomycin D (AMD)and congeners containing c/s-4-chloroproline in place of one or both prolines. In the present study a wider range of naturally occurring actino...

Reversed-phase High-performance Liquid Chromatography and Scanning Electron Microscopy

The zein proteins of eight opaque maize mutants were examined by reversed-phase high-performance liquid chromatography (RP-HPLC) and the microstructure of their endosperms by scanning electron microscopy (SEM). The opaque (0) mutants included 01, 05, 09) 010) 011) hI, Oh43JlI and V\ 64AJll. In these opaque mutant maize kernels, the proportion (%) of ex-zein, the major component of the alcohol-e...